Example of a Microbiology Unknown Lab Report

 

microbiology lab report example

Kimsey Cooper. Microbiology Lab Final Unknown Report Section#/ Radha. Report on Identification of Mixed Culture Unknown Lab Exercise Introduction. The purpose of this experiment is to apply the knowledge gained from the entire semester in the Microbiology Lab and apply it to be able to identify bacteria. Each student was given a tube that had a mixture of two different types of /5(8). HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to. Jun 19,  · Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction. In this paper I will discuss the processes of how I came to find my two unknown bacteria. This will be a vital task to take with me into my profession for many reasons. In the medical field bacteria and infections of different kinds are the core of the practice.


Microbiology Final Laboratory Report | Chemistry | Nature


The purpose of this experiment is to apply the knowledge gained from the entire semester in the Microbiology Lab and apply it to be able to identify bacteria. Each student was given a tube that had microbiology lab report example mixture of two different types of bacteria inside. The tube used in this experiment was tube number fourteen. Inside the tube was one gram negative and one gram positive organism, microbiology lab report example.

The bacteria of which we learned about and of which were possibilities to be inside the test tubes include: Corynebacterium xerosis, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermis, Clostridium perfringens, Enterobacter aerogenes, Pseudomonas aeruginosa, Neisseria flavescens, Proteus vulgaris, and Moraxella catarrhalis.

The organisms in the test tubes must be streaked for isolation for oxygen requirements and followed with additional tests based on results. The first steps taken in lab were to conduct a gram stain from the organisms in the mixed culture using the techniques and steps in exercise seven. Using aseptic techniques from exercise 5 in the lab manual, I then inoculated the mixed culture onto two Tryptic Soy Agar, TSA, plates and incubated them for 24 hours in 37C in both aerobic and anaerobic microbiology lab report example. The next week, I aseptically streaked the mixed culture from the tube again with an inoculating loop onto two different types of agar.

I inoculated both plates for 24 hours in 37C. After that, I aseptically conducted another gram stain test using lab exercise seven to be sure of the morphology of my organisms. The next route I took was for gram positive organisms. For the microbiology lab report example positive organisms I first conducted a Carbohydrate Fermentation and Hydrogen Sulfide production test. I then incubated it for 24 hours at 37C. The next test I conducted was the Catalase test.

I aseptically mixed a bacterial colony onto a clean glass slide with a drop of water. For this test I aseptically streaked for isolation and incubated the agar for 24 hours at 37C.

After that I conducted a Coagulase test. This test used citrated rabbit plasma as the medium, microbiology lab report example. I aseptically inoculated a loop full of the bacteria into the tube and inoculated it for 24 hours in 37C, microbiology lab report example. Finally, the last test I conducted for the gram positive organisms was the DNase test. This test was for my organism because it was Catalase positive.

For this test I aseptically heavily spot-inoculated microbiology lab report example organism onto two methyl green agar plates and incubated for 24 hours in 37C. For my gram negative organisms, I conducted totally different tests.

The first test I conducted was the Oxidase test. For this microbiology lab report example I aseptically transferred an individual bacterial colony onto a glass slide and added a drop of water, and mixed them together.

I then transferred with my loop a small amount onto the oxidase test strip and the results were instantaneous. The first step for this test was to aseptically transfer part of my bacterial colony into a broth tube and incubate for 48 hours at 37C. After that, I transferred 2. I vortexed the solution and added 5 drops of Methyl Red indicator and waited for a color change. This series also included the MRVP test.

First came the Indole test. The next test out of that series I conducted was the Simmons Citrate test. After this test I conducted the Urease test.

For this test I used the urea agar slants. I aseptically streaked the agar on the top of the surface and incubated for 24 hours at 37C. The last test I conducted for my gram negative organism was the Nirtrate Reductase test. For this test I aseptically inoculated the nitrate broth with my organism and incubated the tubes for 48 hours at 37C.

I did not have to add the zinc dust. The results for my gram positive bacteria were first of all, gram positive cocci. This means that after incubation, the slant of the agar turned pink alkalinethe butt of the agar turned yellow microbiology lab report examplemicrobiology lab report example, and the butt of the agar did not have any cracking or any blackening in the medium.

The results for the Catalase test were a bubbling over the bacteria on microbiology lab report example glass slide which means a positive reaction for catalase.

The results for the MSA were negative for mannitol fermentation. The colonies remained translucent, microbiology lab report example. The results for the Coagulase test negative and no clotting was found in the citrated rabbit plasma. There was no zone of clearance around the spot inoculated bacteria. The results for my gram negative bacteria were first of all, microbiology lab report example, gram negative rods.

The results from the MAC agar were negative and I the colonies remained translucent. The results I obtained from the oxidase test were positive, microbiology lab report example.

The results for the Methyl Red test were positive and the medium turned a red color. The results for the Vogues Proskauer test were negative and microbiology lab report example medium did not impart a rose color. The results for the Indole test were positive and the medium turned a cherry red color. The results for the Urease test were positive as well and the agar changed from a salmon-orange to a fuchsia color.

The microbiology lab report example test I conducted was the Nitrate Reductase test. The results for this test were positive as well, microbiology lab report example. The liquid broth turned red and created a red precipitate. I will now discuss the purpose of inoculating all of the tests chosen for my gram positive organism, microbiology lab report example.

The first test conducted was on the CNA agar. This tested for the hemolysis pattern of my organism. The purpose of conducting this test is because it enabled me to differentiate between the degrees of which the hemolysins lysed red blood cells.

The incubation times for all of the organisms for 24 hours and the temperature at 37C is important because this is the optimal temperature to maintain agar state and also the perfect temperature that provides the right conditions for most microbial growth. In these tests, most of the incubation times and temperatures were the same for basically the same reasons.

The second test conducted was on the TSI agar. This agar differentiates between the ability of organisms to ferment glucose, sucrose, or lactose and to liberate hydrogen sulfide gas from sodium thiosulfate.

My gram positive organism was Microbiology lab report example - which meant that the slant was alkaline pinkthe butt was acidic yellowand it did not liberate the gas were no cracks in medium.

These results showed that my organism ferments glucose only. The third test conducted was the Catalase test, microbiology lab report example. This test was to check for the presence of the catalyst catalase which liberates molecular oxygen. This catalyst is necessary for organisms to be able to survive in aerobic conditions. After the experiment, it was seen that my gram positive organism did in fact bubble, and utilize catalase.

The fourth test conducted was on the MSA agar. This test was conducted for the purpose of selective and differential halophiles. It is based on mannitol fermentation. The phenol-red indicator helps to identify the bacteria. My colonies were shown not to ferment mannitol and they remained translucent. The fifth test conducted was the Coagulase test. The coagulase test is important for distinguishing between species of Staphylococci that are catalase positive, which is why I conducted it.

Coagulase is an extracellular protein involved in blood clotting which is why the rabbit plasma was provided as the medium. The plasma is a source of fibrinogen, citrate and EDTA which are needed to form clots. My organism did not form a microbiology lab report example and therefore was negative. This test is also conducted for catalase positive organisms. DNases aid in the spread of bacteria throughout tissues. The soy and peptones in the media provide nutrients and the sodium chloride provides the osmotic equilibrium for this test.

The 0. The DNase positive organisms produce a zone of clearing around the organism. My organism did not produce that and therefore does not contain DNase. Based on all of the results from these tests, my conclusion is that my gram positive cocci organism is, Staphylococcus Epidermis. I will now discuss the purpose of inoculating all of the tests chosen for my gram negative organism. The first test conducted was on the MAC agar. The neutral red indicator serves to contrast the lactose-fermenting from the non lactose-fermenting organisms.

This result means that my organism does not ferment lactose. Microbiology lab report example second test conducted was the Oxidase test. This test was looking for the presence of cytochrome c oxidase which is important in the determination of aerobic conditions. Without this enzyme, an organism cannot survive around oxygen. I observed this reaction so my organism utilizes oxidase and is aerobic. This test differentiates between organisms by detecting fermentative end-products following growth in a buffered peptone-glucose broth.

In the Methyl Red test, the low pH of 4 is indicated by a red medium. My organism tested positive for the mixed-acid fermentation with the red color, but tested negative for the 2,3-butanediol fermentation. This test needed to be incubated for 48 hours instead of 24 hours to allow for extra time for growth of microbiology lab report example bacteria and because it is a broth medium.

 

How to Write a Microbiology Lab Report: 14 Steps (with Pictures)

 

microbiology lab report example

 

Example: 17 bacteria, 2 yeast, and 1 protozoan. If a number starts a sentence spell out the number, do not use a numeral. Example: ten mannitol salt agar plates were streaked Calculations. The equation should be indicated. In a lab report, even if you use a calculator, you must set up the problem. Tables. HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to. Kimsey Cooper. Microbiology Lab Final Unknown Report Section#/ Radha. Report on Identification of Mixed Culture Unknown Lab Exercise Introduction. The purpose of this experiment is to apply the knowledge gained from the entire semester in the Microbiology Lab and apply it to be able to identify bacteria. Each student was given a tube that had a mixture of two different types of /5(8).